Addgene

We narrowed to 23,580 results for: nes

Showing: 221 - 240 of 23580 results

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-cPHx3

Plasmid

#116862

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltNLS/NES-40-mCherry

Plasmid

#234066

Purpose

Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltNLS/NES-40-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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V5-GFP-APEX2-NES_pLX208

Plasmid

#202018

Purpose

expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-GFP-APEX2-NES

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Use

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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m6A-Tracer-NES

Plasmid

#159607

Purpose

Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

m6A-Tracer-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-TurboID-NES_pLX304

Plasmid

#202008

Purpose

expresses TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-TurboID-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Halotag-FRB-EX-nes_pLX304

Plasmid

#120913

Purpose

Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

HA-Halotag-FRB-EX-NES

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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MMLVgag-3NES-Cre

Plasmid

#207252

Purpose

Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MMLVgag-3NES-Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NES

Plasmid

#199545

Purpose

expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NES and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3M14nes

Plasmid

#155367

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-NES

Plasmid

#140901

Purpose

mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NES

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NES (Cytosol)

Plasmid

#220341

Purpose

Mammalian expression vector BS2 in cytoplasm

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Use

Tags

NES (Nuclear exclusion sequence) and V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-Nesprin-1αKASH

Plasmid

#187015

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

Nesprin-1alphaKASH

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pT2ADW-4146NES(ROCK)

Plasmid

#162485

Purpose

For generating transgenic mice expressing ROCK biosensor based on FRET.

Depositor

Michiyuki Matsuda

Article

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ROCK biosensor based on FRET.

Use

Tags

YPet and ECFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSC-NES-RFP

Plasmid

#221812

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NES-mCherry

Use

Lentiviral

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NES-DsRed

Plasmid

#17286

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-NES (Pvalb Rat)

Use

Adenoviral

Tags

DSR and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only