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Plasmid#173455PurposeContains mScarlet-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300
Plasmid#204697PurposeThis plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 to p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hMeikin(1-332)-mNeonGreen
Plasmid#174714PurposeStable expression of fluorescent Separase cleavage sensor with human MeikinDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332PromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept
Plasmid#182424PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteinsDepositorInsertsUseTagsVesicle Nucleating peptide (VNp), and Vesicle Nuc…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mNeongreen-pGBD(PKN1 codon optimized)
Plasmid#129634PurposeVisualization of active RhoADepositorInsertRhoA binding doamin of PKN1(30 - 100) codon optimized
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2
Plasmid#183744PurposeExpresses Ki67 (shuffled Nterm)-mNeonGreenDepositorInsertMKI67 (shuffled Nterm) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationCD domain with shuffled 4 fragments fused to the …PromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen R119A
Plasmid#226572PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationR119APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen D188A
Plasmid#226573PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationD188APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen F191A
Plasmid#226571PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationF191APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant pLV-SigmaR1-mNeonGreen
Plasmid#226569PurposeEncodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)DepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-recipient_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211651PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertGuide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)
Plasmid#204700PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of all TF-bidning domains and HAT in p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)
Plasmid#204699PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of all TF-bidning domains in p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)
Plasmid#204698PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationD1399Y substitution in p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mNeonGreen-stop
Plasmid#186392PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mNeonGreen-RfA
Plasmid#186382PurposeDestination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))
Plasmid#218508Purposeintegrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertmNeongreen
UseTagsmodified ssrA degradation tagExpressionBacterialMutationdeletion of MCS containing Plac_lacZa, introducti…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen
Plasmid#218645PurposeGateway shuttling vector for APEX2-tagged P4C, a P4C biosensorDepositorInsertSidC(P4C)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only