Addgene

We narrowed to 405 results for: lenticrispr

Showing: 221 - 240 of 405 results

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LbCas12a

Plasmid

#188493

Purpose

Expresses FLAG tagged LbCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j3 KRAB

Plasmid

#188507

Purpose

Expresses FLAG tagged dCas12j3 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j2 VPR

Plasmid

#188518

Purpose

Expresses FLAG tagged dCas12j2 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170811

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN3_EF1a-tNGFR-2A-Puro

Plasmid

#201113

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 3

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN4_EF1a-tNGFR-2A-Puro

Plasmid

#201114

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 4

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN5_EF1a-tNGFR-2A-Puro

Plasmid

#201115

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 5

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN6_EF1a-tNGFR-2A-Puro

Plasmid

#201116

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 6

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN7_EF1a-tNGFR-2A-Puro

Plasmid

#201117

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 7

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN8_EF1a-tNGFR-2A-Puro

Plasmid

#201118

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 8

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN1_EF1a-tNGFR-2A-Puro

Plasmid

#200553

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 1

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-bleo-ErbB4

Plasmid

#197353

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e VPR

Plasmid

#188515

Purpose

Expresses FLAG tagged DpbdCas12e VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DpbCas12e

Plasmid

#188495

Purpose

Expresses FLAG tagged DpbCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PlmCas12e

Plasmid

#188496

Purpose

Expresses FLAG tagged PlmCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge