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Showing: 221 - 240 of 21852 results
  1. pSLC-242

    Plasmid
    #73189
    Purpose
    Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Insert
    chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    chloramphenicol promoter
    Available since
    July 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid
    #129538
    Purpose
    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox; Mos1-mediated single copy insertion (mos…
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. mcherry-p62 delta PB1 silent

    Plasmid
    #187985
    Purpose
    human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552
    Depositor
    Insert
    p62 (NUP62 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lacking PB1 domain (aa1-102) with a silent mutati…
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pDule2 - Mj Acd A9

    Plasmid
    #197652
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pDule- Mj Acd A9

    Plasmid
    #197651
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pFa6a MET25

    Plasmid
    #61913
    Purpose
    Expression of S. cerevisiae MET25 cloned into pFA6a
    Depositor
    Insert
    MET25
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pL-Sapphire

    Plasmid
    #176568
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of leucine
    Depositor
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pH-mWasabi

    Plasmid
    #176551
    Purpose
    Expression of mWasabi for auxotrophic selection in the absence of histidine
    Depositor
    Insert
    mWasabi
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pU-mWasabi

    Plasmid
    #176553
    Purpose
    Expression of mWasabi for auxotrophic selection in the absence of uracil
    Depositor
    Insert
    mWasabi
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pM-mWasabi

    Plasmid
    #176554
    Purpose
    Expression of mWasabi for auxotrophic selection in the absence of methionine
    Depositor
    Insert
    mWasabi
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pU-TagRFP657

    Plasmid
    #176562
    Purpose
    Expression of TagRFP657 for auxotrophic selection in the absence of uracil
    Depositor
    Insert
    TagRFP657
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pH-Sapphire

    Plasmid
    #176566
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of lysine
    Depositor
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pU-Sapphire

    Plasmid
    #176567
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of uracil
    Depositor
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pM-Sapphire

    Plasmid
    #176569
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of methionine
    Depositor
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pRG02

    Plasmid
    #44196
    Depositor
    Insert
    Neo
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 21852 results