-
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
UseTagsExpressionBacterialMutationPromoterchloramphenicol promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-LIR-mutant (LIRAAAA)
Plasmid#187981PurposePlasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationDDDW335-338AAAA (LIR mutant), silent mutation siR…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)
Plasmid#129538Purposemodified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selectionDepositorTypeEmpty backboneUseCre/Lox; Mos1-mediated single copy insertion (mos…TagsExpressionWormMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_B1ChR2_TS_mScarlet_ER
Plasmid#195191PurposeHighly K+ selective channelrhodopsinDepositorInsertBilabrum sp. K+ selective channelrhodopsin 2
UseTagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mcherry p62 delta UBA silent
Plasmid#187984Purposemcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationLacking UBA domain (aa389-434) but it has the sil…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 delta PB1 silent
Plasmid#187985Purposehuman p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationLacking PB1 domain (aa1-102) with a silent mutati…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC6 H216A
Plasmid#224351PurposeExpresses human KDAC6 (HDAC6) H216A in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 216 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC6 H611A
Plasmid#224352PurposeExpresses human KDAC6 (HDAC6) H611A in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 611 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFa6a MET25
Plasmid#61913PurposeExpression of S. cerevisiae MET25 cloned into pFA6aDepositorInsertMET25
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL-Sapphire
Plasmid#176568PurposeExpression of Sapphire for auxotrophic selection in the absence of leucineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mWasabi
Plasmid#176551PurposeExpression of mWasabi for auxotrophic selection in the absence of histidineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-mWasabi
Plasmid#176553PurposeExpression of mWasabi for auxotrophic selection in the absence of uracilDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mWasabi
Plasmid#176554PurposeExpression of mWasabi for auxotrophic selection in the absence of methionineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-TagRFP657
Plasmid#176562PurposeExpression of TagRFP657 for auxotrophic selection in the absence of uracilDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-Sapphire
Plasmid#176566PurposeExpression of Sapphire for auxotrophic selection in the absence of lysineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-Sapphire
Plasmid#176567PurposeExpression of Sapphire for auxotrophic selection in the absence of uracilDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-Sapphire
Plasmid#176569PurposeExpression of Sapphire for auxotrophic selection in the absence of methionineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRG02
Plasmid#44196DepositorInsertNeo
UseTagsExpressionWormMutationPromoterrps-27Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only