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Showing: 221 - 240 of 649 results

  2. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

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  3. pCAPB02

    Plasmid
    #65628
    Purpose
    A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis
    Depositor
    Pei-yuan Qian
    Article
    Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    65628-plasmid-map-sequence-id-120067

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  4. pALOD4 non-binding mutant

    Plasmid
    #111027
    Purpose
    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A, G501A, T502A, T503A, L5…
    Promoter
    T7
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111027-plasmid-map-sequence-id-214042

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  5. pcpBpaRS

    Plasmid
    #196484
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (synthetase), Pol III tRNA promoter (tRNA)
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196484-plasmid-map-sequence-id-388864

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  6. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Use
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Phyper spank
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  14. pET11a-DAAT-V33G/T242G

    Plasmid
    #159278
    Purpose
    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan
    Depositor
    Roberto Chica
    Article
    Parmeggiani et al ACS Catalysis 9, 3482–3486.
    Insert
    Engineered d-alanine aminotransferase
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Valine 33 and threonine 242 have been mutated to …
    Promoter
    T7
    Available since
    Sept. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159278-plasmid-map-sequence-id-311390

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Showing: 221 - 240 of 649 results