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Plasmid#104996PurposeThis lentiviral construct delivers hSpCas9 and G418 resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG (AAV9)
Viral Prep#187179-AAV9PurposeReady-to-use AAV9 particles produced from pAAV_hSyn1_nLightG (#187179). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightG plasmid DNA. Synapsin-driven expression of norepinephrine sensor nLightG. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhuman Synapsin-1TagsNoneAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143
Plasmid#69295PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB5
Plasmid#19473DepositorInsertDNAJB5 (DNAJB5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB5
Plasmid#19473DepositorInsertDNAJB5 (DNAJB5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBV514
Plasmid#204602PurposeExpresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoterDepositorInsertOs Tir1 (LOC4335696 Oryza sativa)
UseTagsMyc epitope tagsExpressionBacterialMutationChanged phenylalanine 74 into an alanine (F74A).PromoterScADH1Available sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid#60231PurposeExpresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
EGFP
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA-P2A and EGFP-KASHExpressionMammalianMutationPromoterU6 and hSynAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40
Plasmid#101060PurposeAAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-WT-Highest-CAR_RHL004
Plasmid#164826PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CARDepositorInsertAntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLXSN16E6E7
Plasmid#52394PurposeThe plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.DepositorInsertHPV16 E6E7
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fragmid
Plasmid Kit#1000000237PurposeFragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.DepositorAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-hGSDMD
Plasmid#111559PurposeExpress human GSDMD in E.coliDepositorInsertGasdermin D (GSDMD Human)
UseTagsHis, SUMOExpressionBacterialMutationPromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-B19G (AAV1)
Viral Prep#100799-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.DepositorPromoterTREtightTagsBFP (in presence of Cre and 100798)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV GFP
Plasmid#49055Purposeadeno-associated virus (AAV) encoding a green fluorescent proteinDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-TurboID(full)-HA-GPI
Plasmid#166055PurposeExpresses the full length TurboID on the surface of astrocytesDepositorInsertGPI anchored TurboID
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21∆serB
Bacterial Strain#34929DepositorBacterial ResistanceNoneAvailable sinceFeb. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Eco
Plasmid#35617DepositorInsertEcotropic MLV envelope
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Eco
Plasmid#35617DepositorInsertEcotropic MLV envelope
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only