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Plasmid#163791PurposeThe plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only
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pBS-L1PA8CHmneo
Plasmid#69608PurposeExpresses a neomycin tagged codon optimized L1PA8DepositorInsertL1PA8
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CoV-NSP3-c001
Plasmid#173082PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep
UseTagsmCeruleanExpressionBacterialMutationE1000-K1173, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV LTco S220A
Plasmid#112193PurposeExpression of MCPyV LT mutant (Ser 220 to Ala)DepositorInsertMCPyV LT codon optimized
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_10
Plasmid#60581Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_10
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_11
Plasmid#60582Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_11
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
UseTagsExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A3
Plasmid#132024PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A3 (SLC22A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
P823_eSpCas9(1.1)-RecA
Plasmid#87264PurposeHuman codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiencyDepositorInsertRecA
UseTagsFLAGExpressionMammalianMutationPromoterCBhAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSSM97: pCMV-HA-NLS-SpuFz1-E498R
Plasmid#205275PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationE498RPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM105: pCMV-HA-NLS-SpuFz1-T513K
Plasmid#205276PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationT513KPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM58: pCMV-HA-NLS-SpuFz1-D487K
Plasmid#205274PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD487KPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM59: pCMV-HA-NLS-SpuFz1-D300R
Plasmid#205272PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD300RPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - DDX3Y sgRNA 2
Plasmid#70657PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against DDX3Y
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKDC.015
Plasmid#184992PurposeExpress -Eco2 RT and ncRNA in human cellsDepositorInsertEco2: RT and ncRNA(wt), a1/a2 length: 13
UseTagsExpressionMammalianMutationHuman codon optimized RTPromoterTetOn-3gAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2
Plasmid#127360Purposegateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-N1-iChloC
Plasmid#85467Purposeimproved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)DepositorInsertiChloC
UseTagsmCherryExpressionMammalianMutationE83Q, E90R, E101S, T159C, D156NPromoterCMV (+enhancer)Available sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only