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Showing: 2361 - 2380 of 3974 results

  1. pCEP4-SERTT439M-myc

    Plasmid
    #107466
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T439M
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107466-plasmid-map-sequence-id-205583

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  2. pCEP4-SERTI425L-myc

    Plasmid
    #107465
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425L conferring approximately wildtype transport phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    I425L
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107465-plasmid-map-sequence-id-205576

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  3. pCEP4-SERTG384S-myc

    Plasmid
    #107463
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G384S with loss of surface expression relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G384S
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107463-plasmid-map-sequence-id-205570

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  4. pCEP4-SERTS375L-myc

    Plasmid
    #107462
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S375L
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107462-plasmid-map-sequence-id-205564

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  5. pCEP4-SERTA331T-myc

    Plasmid
    #107461
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A331T
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107461-plasmid-map-sequence-id-205562

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  6. pCEP4-SERTA169D-myc

    Plasmid
    #107460
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A169D
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107460-plasmid-map-sequence-id-205558

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  8. pCMV-tadDE-PcrA M6-UGI

    Plasmid
    #221236
    Purpose
    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.
    Depositor
    Fei Chen
    Article
    Chen et al bioRxiv 2024.02.01.577593
    Insert
    tadDE-PcrA M6-UGI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221236-plasmid-map-sequence-id-437117

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  9. lenti_dCas9-KRAB-MeCP2

    Plasmid
    #122205
    Purpose
    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.
    Depositor
    Andrea Califano
    Article
    Tan et al bioRxiv 2021.06.28.449297
    Insert
    dCas9-KRAB-MeCP2
    Use
    Lentiviral
    Tags
    KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 (D10A, H840A)
    Promoter
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122205-plasmid-map-sequence-id-377395

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  11. piggyFlex

    Plasmid
    #218234
    Purpose
    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
    Depositor
    Jay Shendure
    Article
    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    218234-plasmid-map-sequence-id-432525

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  17. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224898-plasmid-map-sequence-id-443622

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  19. pLV-REPORT(EF1a)

    Plasmid
    #172326
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172326-plasmid-map-sequence-id-354701

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Showing: 2361 - 2380 of 3974 results