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Plasmid#170528PurposeEntry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADMDepositorInsertCavin4 (Cavin4 Mouse)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-MmCav1
Plasmid#170527PurposeEntry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJDepositorInsertCaveolin1 (Cav1 Mouse)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX026
Plasmid#167153PurposeMoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-nnLuz-ocsT
UseLuciferaseTagsHis6-tagExpressionPlantMutationPromoterp35sAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCUU
Plasmid#159747PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GtACR2-ChRmine-tandem-eYFP
Plasmid#192580PurposeAAV-vector for bidirectional optogenetic manipulation of neuronsDepositorInsertsomBiPOLES
UseAAVTagsEYFP- soma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCENPTΔC-dCas9-3xGFP
Plasmid#198325PurposeExpresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locusDepositorInsertCENPTΔC (CENPT Human)
UseLentiviralTagsEGFP x 3 and dCas9ExpressionMammalianMutationtruncation - encodes only amino acids 1-375PromoterCMV-TetOAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-ING2(pHD)
Plasmid#21589DepositorInsertGFP-ING2(pHD) (Ing2 Mouse)
UseTagsEGFPExpressionMammalianMutationcontains Ing2 plant homeodomain (PHD) amino acids…PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA1
Plasmid#98399PurposeLentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
UseTagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-eTLR
Plasmid#214737Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locusDepositorInsertAAVS1-eTLR (PPP1R12C Rabbit, Chicken, CMV, Anguilla japonica, influenza, Synthetic, Bovine, Human)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-EGFP-APEX2 in pCSDEST2
Plasmid#170523PurposeVector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTEDepositorInsertCavin4 (Cavin4 Mouse)
UseTagsAPEX2-P2A-mKate2ExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D122A pcDNA3
Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA E1658A (EIVA) pcDNA3
Plasmid#206114Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B
Plasmid#67523PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
MmCavin4-APEX2-P2A-mKate2
Plasmid#170524PurposeVector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJFDepositorInsertCavin4 (Cavin4 Mouse)
UseTagsAPEX2-P2A-mKate2ExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA2
Plasmid#98400PurposeLentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcdna 3.1 DSG-3 Tailless Control
Plasmid#182718Purposeexpresses a truncated force-insensitive DSG-3 TSmodDepositorInsertdesmoglein-3 (Dsg3 Mouse)
UseTagsExpressionMammalianMutationTSmod inserted at G726, downstream C-terminal tai…PromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVA (E1658A) pCAGGS
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pcDNA3
Plasmid#206066Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only