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Plasmid#195958PurposeGateway pME fluorophoreDepositorInsertmCerulean nuclear
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
opto-Itsn1
Plasmid#174509PurposeExpresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-Itsn1-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
Plasmid#20942Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsExpressionMammalianMutationH134R mutation in ChR2PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
p5E-pth2
Plasmid#191428PurposeTol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)DepositorInsertpth2 regulatory region (pth2 Zebrafish)
UseTol2 gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sp-HPV-TM(ICAM)
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Right DdCBE-G1397-C- T1413I-GFP
Plasmid#179686PurposeExpression of base editor in mammalian cells and for cell sortingDepositorInsertSOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
UseTagsExpressionMammalianMutationT1413IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag
Plasmid#167274PurposeOverexpression of CLIP-tag in E. coliDepositorInsertCLIP-tag
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
ChromosomesMembranesandCytoskeleton
Plasmid#62450PurposeMXS_chaining vector with 4 cassettesDepositorInsertsCMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H2
Plasmid#79513PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDSDepositorInsertLXR BETA (NR1H2 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_HUWE1_isoform1
Plasmid#190464PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BMRF1(EA-D)
Plasmid#195807PurposeGateway Entry Clone of Epstein-Barr virus BMRF1DepositorInsertBMRF1 (BMRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag
Plasmid#167269PurposeOverexpression of SNAP-tag in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ220-Ultra
Plasmid#176889PurposeGateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoterDepositorInsertAsCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
766 p3E-2A-nlsmCherrypA
Plasmid#195972PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsmCherry (nuclear)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only