Addgene

We narrowed to 17,270 results for: gateway

Showing: 2341 - 2360 of 17270 results

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p663-UBC-miniTurbo-V5-AKT1_IDG-K

Plasmid

#189890

Purpose

Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AKT1 (AKT1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12C

Plasmid

#83130

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12C

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12D

Plasmid

#83131

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12D

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b Q61H

Plasmid

#83140

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61H

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLCS104

Plasmid

#209357

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA99

Plasmid

#209334

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12V

Plasmid

#83132

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G13D

Plasmid

#83133

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G13D

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pME-memGFP-P2A

Plasmid

#80809

Purpose

Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-memGFP-P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12R

Plasmid

#83143

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12R

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b Q61L

Plasmid

#83134

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61L

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G13C

Plasmid

#83145

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G13C

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b Q61R

Plasmid

#83135

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61R

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK11B-V5-miniTurbo_IDG-K

Plasmid

#189914

Purpose

Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK11B (CDK11B Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK13_IDG-K

Plasmid

#135266

Purpose

Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK13 (CDK13 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-CDK13_IDG-K

Plasmid

#135267

Purpose

Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-CDK13 (CDK13 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G01

Plasmid

#67135

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDKL5_IDG-K

Plasmid

#170578

Purpose

Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDKL5 (CDKL5 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

E854G- please see depositor comments

Promoter

Ubiquitin

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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