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Plasmid#138293PurposeMammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion proteinDepositorInsertACTN1 (ACTN1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
UseTags3' extension derived from endogenous locus a…ExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO-MESP1
Plasmid#170694Purposedoxycycline-inducible overexpression of MESP1DepositorInsertMESP1 (MESP1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc-mMEKK2
Plasmid#44158DepositorInsertMekk2 (Map3k2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationChanged glutamic acid 36 to lysinePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAL130
Plasmid#22475PurposeExpresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF3NT (PIF3 Mouse, Mustard Weed)
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Myc-huMEKK3 (K391A)
Plasmid#44157DepositorInsertMEKK3 (K391A) (MAP3K3 Human)
UseTagsMycExpressionMammalianMutationK391A, kinase-dead mutantPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-PBD H538A/K540M
Plasmid#60879PurposeBacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutantDepositorInsertPolo-like kinase 1 (PLK1 BC01486, Human)
UseTagsGSTExpressionBacterialMutationH538A/K540MPromoterAvailable sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1
Plasmid#111859PurposeFull length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, TEV site, and ZZExpressionInsectMutationPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTTG-bio-His
Plasmid#51822PurposeExpresses full-length Pituitary tumor-transforming gene 1 protein-interacting protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTTG (PTTG1IP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
tetO-NFYB
Plasmid#170697Purposedoxycycline-inducible overexpression of NFYBDepositorInsertNYFB (NFYB Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700]-Ss(424)
Plasmid#166852PurposeExpresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.DepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeEH258A
Plasmid#129122Purposeexpresses H258A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeES157A
Plasmid#129121Purposeexpresses S157A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
Plasmid#112802PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 14_22 and 447_454 deletedPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2
Plasmid#220243PurposeExpresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only