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Plasmid#206066Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMVtight-PLXNB2 iOE
Plasmid#176849Purposelentiviral vector for doxycycline-inducible PLXNB2 overexpressionDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationfull length PLXNB2PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-hPLXNB2_PGK_Neo
Plasmid#176848Purposelentiviral vector for constitutive PLXNB2 overexpressionDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationfull length PLXNB2PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-Cavin4a-mCherry
Plasmid#176022PurposeLeishmania cell free expression of zebrafish Cavin4a-mCherry. Parton lab clone GWUDepositorInsertCavin4a (cavin4a Zebrafish)
UseLeishmania cell free expressionTagsmCherryExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-Cavin4a-EGFP
Plasmid#176018PurposeLeishmania cell free expression of zebrafish Cavin4a-EGFP. Parton lab clone GRKDepositorInsertCavin4a (cavin4a Zebrafish)
UseLeishmania cell free expressionTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-MmCav1-APEX2
Plasmid#170526PurposeVector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYWDepositorInsertCaveolin1 (Cav1 Mouse)
UseLeishmania cell free expression cloneTagsAPEX2 and LifeactExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)
Plasmid#156417Purpose8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sitesDepositorInsertmurine Dll4 F2-6/F8 ETS site B/ site #2, 8X
UseZebrafish transgenesisTagsExpressionMutationPromoterE1b min pro/b-globin intronAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Tomato
Plasmid#214403PurposeExpresses human SOX10, OLIG2, NKX6.2 and tdTomatoDepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-Tet-O-RUNX1T1-Puro
Plasmid#221666PurposeExpresses RUNX1T1 in mammalian cellsDepositorInsertRUNX1T1 (RUNX1T1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-puro
Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 RBD (bio-His)
Plasmid#166857PurposeHuman expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.DepositorInsertSARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
UseTags6xHis and Biotinylation siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseGateway cloningTagsEYFPExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-E (P440E)
Plasmid#119978PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.DepositorInsertFusion Glycoprotein type E (P440E)
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 HexaPro spike (bio-His)
Plasmid#166856PurposeHuman expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.DepositorInsertSARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
UseTags8xHis and Biotinylation siteExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-Caveolin1-CMV-GFP
Plasmid#83272PurposeAdenoviral expression of Caveolin1 with co-expression of GFPDepositorInsertsUseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 GBP (GBP-mEos2)
Plasmid#162876PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-E63-puro
Plasmid#156167PurposeLentiviral vector for expression of RAP1A-E63, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Glutamine 63 to Glutamic acidPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only