Addgene

We narrowed to 3,401 results for: mak

Showing: 2321 - 2340 of 3401 results

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pX330-Elf5 gRNA

Plasmid

#128836

Purpose

gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

mElf5 gRNA (Elf5 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Eomes

Plasmid

#128825

Purpose

dox-dependent expression of mouse Eomes

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Eomes (Eomes Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Shorter isoform

Promoter

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-proα1(I)

Plasmid

#119844

Purpose

Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α1 chain (Col1a1 Mouse)

Use

Tags

mApple

Expression

Mammalian

Mutation

Col1a1 exons 2-3 replaced by fluorescent tag

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata3

Plasmid

#128824

Purpose

dox-dependent expression of mouse Gata3

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Gata3 (Gata3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS6-mVenus

Plasmid

#185806

Purpose

NDUFS6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS6 (NDUFS6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Klf5

Plasmid

#128830

Purpose

dox-dependent expression of mouse Klf5

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Klf5 (Klf5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon/Von GCaMP 6m

Plasmid

#137165

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

3x-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

Ef1a

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-BFP

Plasmid

#137130

Purpose

Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-eYFP-WPRE

Plasmid

#183530

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-eYFP

Use

AAV

Tags

Expression

Mutation

H33R

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mIFP-MAPTau-N-10

Plasmid

#56230

Purpose

Localization: Microtubules, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MAPTau (MAPT Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mApple-proα2(I)

Plasmid

#119840

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

mApple

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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mIFP12-Farnesyl-5

Plasmid

#56251

Purpose

Localization: Plasma Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Farnesyl (HRAS Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP12-Fibrillarin-7

Plasmid

#56252

Purpose

Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Fibrillarin (FBL Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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FLAG-HA-FbxO11-Jeff-pcDNA3.1-

Plasmid

#52503

Purpose

expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Q491L (Jeff)

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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mVenus-proα2(I)

Plasmid

#119838

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mIFP-EB3-7

Plasmid

#56218

Purpose

Localization: MT End Binding Protein, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

EB3 (MAPRE3 Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits