-
Plasmid#194071Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-BirA(R118G)-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pEF 3-CFP-2
Plasmid#20105DepositorInsertCFP (Cfp )
UseSynthetic Biology; Plant gateway vectorTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mTagBFP2 L1-R5
Plasmid#84388PurposeMultisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmTagBFP2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREP4xN_ccdB
Plasmid#79022PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xN_ccdB
Plasmid#79026PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xN_ccdB
Plasmid#79025PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)
Plasmid#228043PurposeDONR vector for inflammasome speck reporter, designed from PMC9623368.DepositorInsertCASP1[CARD]-mSG2
UseGateway donrTagsExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 Rab25-STOP
Plasmid#228034PurposeDONR vector for shuttling Rab25 into DEST vector. Contains a STOP codon.DepositorInsertRab25 (RAB25 Human)
UseGateway donrTagsExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pENTR CMVie-Lifeact-mCardinal L1-R5
Plasmid#84392PurposeMultisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmCardinalExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREP41xN_ccdB
Plasmid#79023PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xN_ccdB
Plasmid#79024PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E_unc503
Plasmid#64020Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bDepositorInsertmuscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b S17N
Plasmid#83156PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationS17NPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG- gate-pGK-HYG
Plasmid#107397PurposeGateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Tp-SceI-pheS
Plasmid#111608PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-AKT1-V5-miniTurbo_IDG-K
Plasmid#189889PurposeGateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only