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Plasmid#62865PurposeYeast two-hybrid bridge vector expressing PAA-1DepositorInsertPAA-1/Y3H
UseTwo-hybrid bridge vectorTagsExpressionYeastMutationPromoterTEFAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCeSR beta deltaN
Plasmid#13728DepositorInsertSignal recognition particle receptor β subunit (R186.3 Nematode)
UseTags6xHisExpressionBacterialMutationexpresses residues 38-240 of C. elegans SRβ with …PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pScSR beta deltaN Flag
Plasmid#13729DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHis and FlagExpressionBacterialMutationexpresses residues 31-244 of S. cerevisiae SRβ wi…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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RBMS2_2xRRM_pGEX
Plasmid#135132PurposeRecombinant expression of RBMS2 with dual-affinity tagDepositorInsertRBMS2 (RBMS2 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(L)Cas9
Plasmid#127385PurposeExpresses Cas9 at low levelDepositorInsertuORF(L)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAS9PBKS
Plasmid#68371PurposeCbAkt promoter driven Cas9-2A-GFP (from px458)DepositorInsertCas9
UseTags2A-EGFPExpressionMammalianMutationPromoterCbAktAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-ultraID
Plasmid#172878Purposeexpresses ultraID in mammalian cells with the tet-on or tet-off systemDepositorInsertultraID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21 (ORF-EG)
Plasmid#46948PurposeInducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Hgpm2
Plasmid#164441PurposeEncodes codon-optimized HIV gagpol for pseudovirus productionDepositorInsertCodon Optimized HIV-1 GagPol
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorInsertGFP-RelA (RELA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657
Plasmid#212961PurposeLentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_backbone
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pRC-CMV-Rev1b
Plasmid#164443PurposeEncodes HIV-1 Rev accessory protein for pseudovirus productionDepositorInsertHIV-1 Rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Luc2-IRES-ZsGreen-W
Plasmid#164432PurposeLentiviral vector backbone encoding firefly luciferase and ZsGreenDepositorInsertsLuciferase
ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCBP1_Full-length_pGEX
Plasmid#135108PurposeRecombinant expression of PCBP1 with dual-affinity tagDepositorInsertPCBP1 (PCBP1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only