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Plasmid#176732PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).DepositorArticleInsertste2Tp
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm) -R
Plasmid#48888PurposeNegative feedback plasmid from oscillator without luxRDepositorInsertaiiA
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLP-hSox2-ires-gfp
Plasmid#49391Purposemammalian bicistronic expression of human Sox2 and EGFPDepositorInsertSox2 (SOX2 Human)
UseCre/Lox; Acceptor vectorTags6xHN, EGFP, and IRESExpressionMammalianMutationTGA (stop) to CCA (Pro) at nt 1027 with respect t…PromoterCMVAvailable sinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAndy-CBioTAP
Plasmid#53570PurposePlasmid vector carrying C-Bio-1xproteinA tagDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDXNSM3
Plasmid#49028PurposeFX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), MYC, and str…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-134
Plasmid#185925PurposeHimar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein geneDepositorInsertmOrange2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKR2.40
Plasmid#26952DepositorInsertpie-1promoter-GFP- gateway destination cassette B -pie-1 3’
UseTagsGFPExpressionWormMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
LIIbeta F 1-2
Plasmid#54356DepositorTypeEmpty backboneUsePlant expressionTagsExpressionMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2112
Plasmid#141296PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDCAF-ABC
Plasmid#113652PurposeDemethylates methylxanthines.DepositorInsertndmA and ndmB and ndmC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPSSUB-101
Plasmid#26580DepositorInsertdestabilized GFP
UseTagsExpressionBacterialMutationS2R, C-terminal extension RPAANDENYA(LVA)PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYEXNH3
Plasmid#49024PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionYeastMutationPromoterGAL1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b
Plasmid#164862PurposeInducible expression of BzCas13b nuclease in bacteria.DepositorInsertBergeyella zoohelcum Cas13b
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-676 (attR4-attR3)
Plasmid#161888PurposeMultisite Gateway (attR4-attR3) vector for lentivirus productionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-686 (attR4-attR2)
Plasmid#161889PurposeMultisite Gateway (attR4-attR2) vector for lentivirus productionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP
Plasmid#72947PurposeBackbone vector with internal BsmBI removed by site-directed mutagenesisDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Destination Vector
Plasmid#125754PurposeDestination vector for assembling gRNA fragments in the second round of assembly.DepositorInsertpTDH3-GFPdropout-tTDH1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Guide-Generating Vector
Plasmid#125752PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assemblyDepositorInsertCys4-GFPdropout-SpCas9scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
LIIbeta R 3-4
Plasmid#54361DepositorTypeEmpty backboneUsePlant expressionTagsExpressionMutationPromoterAvailable sinceJune 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDest-305 (attR4-attR2)
Plasmid#161895PurposeMultisite Gateway (attR4-attR2) vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only