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VAMP2-pHmScarlet

Plasmid

#166890

Purpose

For vesicle exocytosis visualization using pHmScarlet

Depositor

Pingyong Xu

Article

Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

Insert

VAMP2 (Vamp2 Mouse)

Tags

pHmScarlet

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV8)

Viral Prep

#50476-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

CaMKII

Tags

mCherry

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAT9752-BEAR-mScarlet

Plasmid

#162991

Purpose

BEAR target plasmid with split mScarlet and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pET22b_EcSSB_1

Plasmid

#78203

Purpose

Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

Depositor

Martin Webb

Article

Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

Insert

ssb (G26C)

Expression

Bacterial

Mutation

G26C (please see depositor comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-GFP

Plasmid

#124770

Purpose

3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV1)

Viral Prep

#50477-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

CaMKII

Tags

mCherry

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only
pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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sfGFP-N1

Plasmid

#54737

Purpose

Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Article

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

sfGFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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LI Intron c1

Plasmid

#54433

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Intron element for construction of silencing vectors (intron1 of AtWRKY33)

Use

Cloning vector

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(wt)--2A-mCherry-puro

Plasmid

#231974

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pJeM1

Plasmid

#135088

Purpose

rhamnose-inducible expression vector for replication in E. coli and P. putida

Depositor

Josef Altenbuchner

Article

Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

Insert

rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC81-10XUAS-IVS-Syn21-GFP-p10

Plasmid

#36432

Depositor

Gerald Rubin

Article

Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

Insert

GFP

Expression

Insect

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

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pBP2MF

Plasmid

#31279

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Per2 (Per2 Mouse)

Tags

Flag and Myc

Expression

Insect

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pGL4-UPRE-luc2P-Hygro

Plasmid

#101788

Purpose

Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xUPRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBoxCDGC-KMet-EGFP

Plasmid

#140272

Purpose

L7Ae-dependent translational repression

Depositor

Hirohide Saito

Article

Saito et al Nat Chem Biol. 2010 Jan;6(1):71-8. doi:

Insert

BoxCD

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GPR50-Tango

Plasmid

#66361

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR50 (GPR50 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Promoter

NF-kappaB binding site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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