168,871 results

1. p65-mNeonGreen

   Plasmid

   #127172

   Purpose

   Fluorescently tagged p65 reporter

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   GFP-RelA (RELA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCP-M-MLV RT∆RNase H

   Plasmid

   #213749

   Purpose

   For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ-dzCas9-Act3.0

   Plasmid

   #158414

   Purpose

   CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-G3BP1-WT

   Plasmid

   #135997

   Purpose

   WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK172 asyn(1-122)

   Plasmid

   #240598

   Purpose

   Overexpress truncated alpha-Synuclein protein

   Depositor

   Jennifer Lee

   Article

   Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

   Insert

   aSyn (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   aa 1-122 only

   Promoter

   T7

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-29b(+)_NSP4ss_SerM_NucA-His

   Plasmid

   #218190

   Purpose

   Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Tags

   His and NSP4 signal secretion peptide

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-tdTomato (AAV PHP.S)

   Viral Prep

   #28306-PHP.S

   Purpose

   Ready-to-use AAV PHP.S particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CaMKII-PinkyCaMP

   Plasmid

   #232858

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMG-RIS

    Plasmid

    #225132

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLV-TetO-hNGN2-Puro

    Plasmid

    #79049

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Promoter

    TetO

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRESTO-Tango Kit

    Plasmid Kit

    #1000000068

    Purpose

    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.

    Depositor

    Bryan Roth

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

14. mTurquoise2-pBAD

    Plasmid

    #54844

    Purpose

    Localization: Protein Expression Vector , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    6x His, T7 tag, and TEV site

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral Prep

    #137141-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Flp-dependent)

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-C-terminal GST

    Plasmid

    #128025

    Purpose

    Mammalian expression vector with C terminal GST tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG_eGCaMP(2+)

    Plasmid

    #201148

    Purpose

    expresses eGCaMP2+ in mammalian cells

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP2+
    

    Expression

    Mammalian

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBI-CMV1-mir451-CMV2-mir1

    Plasmid

    #164635

    Purpose

    Express miR-451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR451a (MIR451A Human)
    miR-1-1 (MIR1-1 )
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit

    #1000000024

    Purpose

    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.

    Depositor

    Adam Bogdanove, Daniel Voytas

    Available Since

    Jan. 8, 2013

    Availability

    Academic Institutions and Nonprofits only