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Plasmid#190476Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTRIP-hPGK-Blast-2A-STING-TurboID
Plasmid#204713PurposeExpresses C-terminal TurboID tagged human STING for proximity ligation.DepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationPromoterhPGKAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-P29S
Plasmid#128581PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTags2xMyc epitopeExpressionMammalianMutationChanged Proline29 to Serine.PromoterAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-A KD/MD
Plasmid#104449PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein A (VAPA Human)
UseTagsEGFPExpressionMammalianMutationchanged K94 to D and M96 to DPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B KD/MD
Plasmid#104450PurposeExpression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
UseTagsEGFP and HAExpressionMammalianMutationchanged K87 to D and M89 to DPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-UbC-Blast-2A-STING-mNeonGreen
Plasmid#227185PurposeLentiviral expression plasmid encoding STING-mNeonGreen under UbC promoterDepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterUbCAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1-S127A/S397A
Plasmid#213585PurposeDoxycycline inducible expression of YAP1-S127A/S397A cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationS127A, S397APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1-S127A/S397A/S94A
Plasmid#213586PurposeDoxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationS127A, S397A, S94APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationc.1853A>GPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-IL6_3UTR-xrRNA-4H
Plasmid#108373PurposeExpresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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retro-gfp-puro vector
Plasmid#58249PurposeRetroviral expression vector encoding bicistronic EGFP and Puromycin cassetteDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304-BCL-XL-Y195F
Plasmid#203573PurposeExpresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.DepositorInsertBCL2L1 (BCL2L1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationChanged Tyrosine 195 to Phenylalanine for partial…PromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-TAZ-S89A/S311A/S51A
Plasmid#213590PurposeDoxycycline inducible expression of TAZ-S89A/S311A/S51A cDNA in mammalian cellsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTags2xHAExpressionMammalianMutationS89A, S311A, S51APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyTagsExpressionInsect and MammalianMutationPromoterEF1aAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-Q61L
Plasmid#128582PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTags2xMyc epitopeExpressionMammalianMutationChanged Glutamine61 to Leucine.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEAHISMRSA2676
Plasmid#96970Purposeexpression of methicillin-resistant Staphylococcus aureus orf 2676DepositorInsertSar2676
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only