Addgene

We narrowed to 8,045 results for: sgRNA

Showing: 2301 - 2320 of 8045 results

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pLentiCRISPR-v1-sgMETAP1_2

Plasmid

#163463

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting METAP1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting METAP1 (METAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGPT2_5

Plasmid

#163455

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 5 targeting GPT2 (GPT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000562805)

Plasmid

#80035

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgMyc_1

Plasmid

#138324

Purpose

Express guide RNA 2 for mouse Myc

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.PAC

Plasmid

#57829

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-Gsk3b(new)

Plasmid

#122341

Purpose

Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Gsk3b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgMyc_2

Plasmid

#138346

Purpose

Express guide RNA 1 for mouse Myc

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-rac2-guides

Plasmid

#168241

Purpose

"neutrophil specific GFP with ubiquitous rac2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

rac2 sgRNAs

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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eSpCas9(1.1)_No_FLAG_LMNA_G2

Plasmid

#178090

Purpose

Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dSpCas9-HF1

Plasmid

#92115

Purpose

Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9-HF1 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, Q926A

Promoter

Cbh

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP1

Plasmid

#75163

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIPk1

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIPK1 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHelper-T7IS1

Plasmid

#140631

Purpose

Expresses ShCAST under the T7 promoter and an empty sgRNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

Plasmid

#140205

Purpose

Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Inserts

mCherry
sgRNA E1

Use

CRISPR and Synthetic Biology; Proof-of-concept te…

Tags

Expression

Mutation

G174D

Promoter

PelcA and U3

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSLiP-G2-RacE

Plasmid

#188966

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: gttagacgctgattacatggactagg
racE

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC42

Plasmid

#66564

Purpose

sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1XPP7
PCP-VP64 IRES mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-cdk2-guides

Plasmid

#168250

Purpose

"neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

cdk2 sgRNAs

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v4

Plasmid

#158032

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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