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Plasmid#206222PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only
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mc2 puro EF1a
Plasmid#206221PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 hygro
Plasmid#206216PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
UseTagsExpressionBacterialMutationGvpA T6A-L40APromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT021-LAT_HASA-HaloTag-3x FLAG
Plasmid#202505PurposeExpress high ASA Lat mutantDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationSeveral leucine subsitutions in transmembrane dom…PromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT020-LAT_dCore-HaloTag-3x FLAG
Plasmid#202504PurposeExpress dCore Lat mutantDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationTruncated transmembrane domainPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT019-LAT_C26A-HaloTag-3x FLAG
Plasmid#202503PurposeExpress C26A Lat mutantDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationC26APromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GRE
Plasmid#172336PurposeGal4 Response elementDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TEG +Q111T
Plasmid#187435PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged glutamine at 117 to threoninePromoterPH and p10Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-MED6-mEmerald donor
Plasmid#197865PurposeDonor plasmid used for building MED6-mEmerald knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA
Plasmid#194725Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFBDepositorArticleUseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y
Plasmid#191459PurposeCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent proteinDepositorInsertmTurquoise2_T203Y
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRuby3 only VLP
Plasmid#182429PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-mRuby3
deltaVP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D5:Luc
Plasmid#193007PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D5)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D5PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D4:Luc
Plasmid#193006PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D4)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D4PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D6:Luc
Plasmid#193008PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D6)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D6PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D1:Luc
Plasmid#193003PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D1)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D1PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only