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pCIneo-lambdaN-HA-HsAGO2-F505V_F

Plasmid

#146289

Purpose

Mammalian Expression of HsAGO2-F505V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F505V (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2-F470V_E

Plasmid

#146225

Purpose

Insect Expression of HsAGO2-F470V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F470V (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2-F505V_E

Plasmid

#146226

Purpose

Insect Expression of HsAGO2-F505V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F505V (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

sequence differs in the first 7 AA from the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9

Plasmid

#188076

Purpose

Recombinant overexpression of human ATG9 in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox5-PLAT-EGFP-6H

Plasmid

#187189

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000057273 (alox5a Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2_E

Plasmid

#146224

Purpose

Insect Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

Contains a K660E mutation compared to the sequenc…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F

Plasmid

#146286

Purpose

Insect Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F2V2 (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

Contains F470V, F505V, K660E mutations compared t…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB

Plasmid

#180197

Purpose

Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-1224

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hSPATA33-8xHIS-1D4

Plasmid

#179133

Purpose

Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (SPATA33 Human)

Use

Tags

1D4 and 8XHIS

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_KLF12

Plasmid

#167767

Purpose

Expresses KLF12 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

KLF12 (KLF12 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR2-IR2 GFP pZa

Plasmid

#170083

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PdnaK-IR2-IR2

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F405G

Plasmid

#169956

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#3

Plasmid

#169791

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#2

Plasmid

#169790

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Grin1 gRNA#1

Plasmid

#169789

Purpose

Cas9-mediated knockout of Grin1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Grin1 (Grin1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND L370G

Plasmid

#169970

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

L370G

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND L368G

Plasmid

#169969

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

L368G

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

Plasmid

#169960

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265C

Plasmid

#164715

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible haid-cbe_v01 entry clo…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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