We narrowed to 8,842 results for: sgRNA

1. pZR060_CROPseq-sgCtrl-CRISPRi

   Plasmid

   #180269

   Purpose

   Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   No-Target Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-TJP1

   Plasmid

   #227299

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM113

   Plasmid

   #127210

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   WUS2, AtSTM, sgRNA, Luc
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYC2085

   Plasmid

   #158721

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP8823

   Plasmid

   #220901

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1071-Lenti-Single loxP

   Plasmid

   #217892

   Purpose

   Lentiviral vector, entry vector for Lentiviral Switch-OVER

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Expression

   Mammalian

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZR064_CROPseq-sgCD4-CRISPRi

    Plasmid

    #180270

    Purpose

    Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    CD4 sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLG_GI2

    Plasmid

    #111593

    Purpose

    mU6-sgRNA GI Library vector

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLG_GI3

    Plasmid

    #111594

    Purpose

    hU6-sgRNA GI Library vector

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJZC523

    Plasmid

    #62320

    Purpose

    sgRNA for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_ATP1A1_G7

    Plasmid

    #173204

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + SpCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EDCPV PCSK5_sg09

    Plasmid

    #232455

    Purpose

    Lentiviral, CRISPR sgRNA for PCSK5

    Depositor

    Kevin Janes

    Article

    Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

    Insert

    PCSK5 sgRNA (PCSK5 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKO024.306-107

    Plasmid

    #242927

    Purpose

    Expression of a Sth1-dCas9 compatible MS2 scRNA J306 and a Spy-dCas9 compatible sgRNA J107

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    sgRNA J107
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Bba_J23119

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCY5h

    Plasmid

    #160295

    Purpose

    Yeast CRISPR plasmid targeting the hphMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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