-
Plasmid#191282PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM3110
Plasmid#40247DepositorInsertattP Site
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pREXC3H
Plasmid#47078PurposeFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tagsDepositorTypeEmpty backboneUseLactococcus lactis expressionTags3C protease site (PreScission site) and decaHis (…ExpressionMutationPromoterNIS (nisin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGC4593
Plasmid#110206PurposeAccessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)DepositorInsertsTEV protease
aaRS
UseTagsExpressionBacterialMutationPromoterPbad and PtetAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP8A-ccdB
Plasmid#83883PurposeDestination vector for integration at HIS3, expression from the ADH1 promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1212
Plasmid#141299PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC-RLUC
Plasmid#98142PurposeGateway compatible construct for N' terminal fusion or promoter driven renilla luciferase reporter, hygromycin resistance cassetteDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsHAExpressionMutationPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS007
Plasmid#164868PurposeThis plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.DepositorInsertphiKO2 operators
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW213ara-hrpS
Plasmid#61435PurposepBAD18-cm encoding rbs33-hrpS-terDepositorInsertrbs33-hrpS-ter
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB168
Plasmid#101671PurposeOpto-T7RNAP*(563-F1), equal expr.DepositorInsertOpto-T7RNAP*(563-F1), equal expr.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2_136
Plasmid#199083PurposeEven ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_137
Plasmid#199084PurposeEven ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ606 - pDESTR4-R3(ttTi14024, X)
Plasmid#34862DepositorInsertttTi14024 targeting
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceJune 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJF104B
Plasmid#113324PurposepTet-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I2R
Plasmid#125064PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to chromosome V: 23743..21744
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I2L
Plasmid#125031PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to chromosome V: 23743..21744
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P*-R
Plasmid#189850PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP*-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP* (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P**-R
Plasmid#189851PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP**-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP** (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R*
Plasmid#189852PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R*-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-P-R
Plasmid#189853PurposeCre-inducible activation of cat for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-cat
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only