Addgene

We narrowed to 3,036 results for: aaas

Showing: 2261 - 2280 of 3036 results

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LENTICRISPR-PGM2_sgRNA1

Plasmid

#201616

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

PGM2 (PGM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TKT_sgRNA2

Plasmid

#201625

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TKT_sgRNA1

Plasmid

#201624

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, L177D

Plasmid

#180649

Purpose

Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, L177D mutation, has siRNA resistan…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgVRK1 #4-Blast

Plasmid

#199647

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mMyh9 - 2

Plasmid

#198492

Purpose

lentiviral stable expression of mMyh9 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mMyh9 (Myh9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 2

Plasmid

#193607

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 2 (INTS12 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 1

Plasmid

#193606

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 1 (INTS12 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-2

Plasmid

#180392

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-2 (Stk16 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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bu6-sgCebpa_v1-mU6-sgCebpb_v1-hU6-sgCebpd_v1

Plasmid

#177257

Purpose

Expresses Cebpa_v1 (bU6), Cebpb_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v1/sgCebpb_v1/sgCebpd_v1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

bU6/mU6/hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-2

Plasmid

#124475

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-2_gRNA (BLK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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VIR_sgRNA

Plasmid

#186661

Purpose

VIR sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

VIR sgRNA Plasmid (vir Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA2

Plasmid

#185007

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.3#3/Cre

Plasmid

#173662

Purpose

Expresses a NT.3-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182380

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mito-mTFP1

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

CMV promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1

Plasmid

#182378

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mito-mTFP1

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

CMV promoter and SV40 promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge