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Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_S1966C
Plasmid#98681PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationS1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-XbaI
Plasmid#87328PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - MPX
Plasmid#89619Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertMitocondrial Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CMV.Pro
Plasmid#79486PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoterDepositorInsertCMV Promoter
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCMV PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-XhoI
Plasmid#87330PurposeTo express shRNA against human Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iR
Plasmid#83314PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iR
UseTags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iR betwe…PromoterT5Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_DUM1B01
Plasmid#71089PurposeGateway entry cloneDepositorInsertClk (Clk Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK278
Plasmid#71134PurposeExpression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to asparagine; additional N-…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK130
Plasmid#71129PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsHis6ExpressionBacterialMutationchanged valine-319 to phenylalaninePromoterT7Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK173
Plasmid#71831PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2C10
Plasmid#70927PurposeGateway entry cloneDepositorInsertokr (okr Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK320
Plasmid#71144PurposeExpression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to serine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK319
Plasmid#71143PurposeExpression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to alanine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK318
Plasmid#71142PurposeExpression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to glutamine; additional N-t…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK315
Plasmid#71141PurposeExpression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to glycine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only