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Addgene
Showing: 2241 - 2260 of 16061 results

  1. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Expression
    Mutation
    Promoter
    GAL1
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

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  2. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Availability
    Academic Institutions and Nonprofits only
    73596-plasmid-map-sequence-id-139406

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  4. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  5. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid
    #44085
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    44085-plasmid-map-sequence-id-61696

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  7. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid
    #44075
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    44075-plasmid-map-sequence-id-61677

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  9. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid
    #44078
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    44078-plasmid-map-sequence-id-61691

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  10. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

    Plasmid
    #44081
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    44081-plasmid-map-sequence-id-61675

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  12. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  17. pEB018

    Plasmid
    #215568
    Purpose
    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus
    Depositor
    Robin Ohm
    Insert
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    Use
    Fungal homologous recombination plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215568-plasmid-map-sequence-id-428736

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Showing: 2241 - 2260 of 16061 results