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Addgene

pEB018
(Plasmid #215568)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215568 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPV033
  • Backbone manufacturer
    Dr. Peter Jan Vonk
  • Backbone size w/o insert (bp) 7568
  • Total vector size (bp) 8362
  • Vector type
    Fungal homologous recombination plasmid
  • Selectable markers
    Nourseothricin, Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
  • Species
    Synthetic; Schizophyllum commune
  • Insert Size (bp)
    794
  • GenBank ID
    XM_003029710

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BcuI (SpeI) site to integrate your construct of interest (e.g. promoter + gene) upstream of the HA-tag for integration of the construct at the targeted integration locus in S. commune. Use this approach to complement a gene knockout, combined with the possibility to subsequently perform pull-down experiments like Western Blotting or ChIP-Seq.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB018 was a gift from Robin Ohm (Addgene plasmid # 215568 ; http://n2t.net/addgene:215568 ; RRID:Addgene_215568)