Addgene

We narrowed to 6,220 results for: mag

Showing: 2221 - 2240 of 6220 results

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ubc-nls-pcp-10xSunTag (SRv1.1)

Plasmid

#185798

Purpose

pcp-10xSunTag component for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-10xSunTag

Use

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-12xSunTag (SRv1.2)

Plasmid

#185799

Purpose

pcp-12xSunTag component for SunRISER SRv1.2

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-12xSunTag

Use

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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miniCMV-iRFP670-tDeg

Plasmid

#185403

Purpose

iRFP670-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

iRFP670-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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miniCMV-mCerulean-tDeg

Plasmid

#185401

Purpose

mCerulean-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

mCerulean-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-MiniE-mEm

Plasmid

#181991

Purpose

MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniE-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

Plasmid

#174026

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-QF2

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9_CL

Plasmid

#166245

Purpose

regulated dCas9 generator

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

P112-sgRNA-term-P104-RBS1-dCas9-B15

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ec-TTL-P112 and Ec-TTL-P104

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ec-TTL-P112 and BBa_J23116

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Tags

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Tags

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pVW294

Plasmid

#139491

Purpose

Insertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGRE2 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pGRE2

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVW295

Plasmid

#139492

Purpose

Insertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pALD3 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pALD3

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSP266

Plasmid

#139488

Purpose

Insertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pCTT1 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pCTT1

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP264

Plasmid

#139487

Purpose

Insertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW200

Plasmid

#139489

Purpose

Insertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD1 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pGPD1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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