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Showing: 2221 - 2240 of 3090 results

  2. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Bing Yang
    Article
    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Tags
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213049-plasmid-map-sequence-id-422145

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  3. pRDA_526

    Plasmid
    #184859
    Purpose
    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    184859-plasmid-map-sequence-id-362663

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  4. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Use
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

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  13. ATP1A1_G2_Dual_sgRNA

    Plasmid
    #173201
    Purpose
    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173201-plasmid-map-sequence-id-339508

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  19. pJJR83

    Plasmid
    #75028
    Purpose
    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Article
    Ramalho et al Development. 2020 Jul 22;147(14):dev1880
    Insert
    mCherry^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized mCherry
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75028-plasmid-map-sequence-id-141918

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Showing: 2221 - 2240 of 3090 results