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Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pTH101
Plasmid#84459PurposepJB38-NWMN29-30 + SarA_P1-DsRed-TermDepositorInsertSarA_P1-DsRED from pTH1
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGZ001
Plasmid#48868PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP2832
Plasmid#160672PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2820
Plasmid#160669PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD33-mf-lon
Plasmid#21867DepositorInsertLon (Mfl404 Mesoplasma florum)
UseTagsExpressionBacterialMutationall TGA codons changed to TGG codonsPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEcoFus
Plasmid#187833Purpose1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[himar1C9]
Plasmid#213906PurposeMariner transposon with ATc-inducible himar1C9 transposaseDepositorInsertmariner transposon with cam resistance and ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R
Plasmid#189849PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1L
Plasmid#125030PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3L
Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest-623 (attR4-attR3)
Plasmid#161878PurposeMultisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
psgRNAc
Plasmid#114006Purposeexpress sgRNA, p15A, CRMDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCACTUS
Plasmid#167559PurposeSuicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.DepositorTypeEmpty backboneUseSuicide cloning vector, temperature sensitive.TagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHN1_lacUV5-mNG-PDT
Plasmid#165583PurposeExpresses mNeonGreen and C-terminally fused PDTDepositorInsertmNeonGreen-PDT
UseTagsProtein degradation tagExpressionBacterialMutationPromoterPtrcAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN112
Plasmid#84464PurposepJB38-NWMN29-30+ SarA_P1-mAmetrine-TermDepositorInsertSarA_P1-mAmetrine from pRN12
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL0_65 [StayGold]
Plasmid#198976PurposeLevel 0 partDepositorInsertStayGold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only