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We narrowed to 3,974 results for: biorxiv

Showing: 2221 - 2240 of 3974 results

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pLenti-rTetR(SE)-VP64-2A-mCherryBSD

Plasmid

#225695

Purpose

Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-VP64

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225926

Purpose

Expressing ACC-1 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

ACC-1 (acc-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

tdc-1

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC1-pA

Plasmid

#220765

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

oScarlert-WPRE-BC1

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

Plasmid

#226516

Purpose

Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

Depositor

Kai Johnsson

Article

Kühn et al bioRxiv 2024.08.28.610127

Insert

TOMM20-SNAP-tag2-T2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225925

Purpose

Expressing LGC-47 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

tdc-1

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TERF1

Plasmid

#222542

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

TERF1 (TERF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP-Cav1-His6

Plasmid

#223700

Purpose

To produce standard caveospheres (without the Z-domain), Parton Lab clone LDB

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP-Cav1-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP-Z-Cav1-His6

Plasmid

#223701

Purpose

To produce z-spheres (caveospheres containing the anti-IgG Z-domain of protein A), Parton Lab clone LCU

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP-Z-Cav1-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB402SC

Plasmid

#224552

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Article

Lawson et al bioRxiv 2024.07.14.603419

Type

Empty backbone

Use

Tags

Twin-Strep-HA

Expression

Plant

Mutation

Promoter

2 X 35S

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB402SN

Plasmid

#224551

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Article

Lawson et al bioRxiv 2024.07.14.603419

Type

Empty backbone

Use

Tags

HA-Twin-Strep

Expression

Plant

Mutation

Promoter

2 X 35S

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hDlx_DIO_ChR2_mCherry

Plasmid

#224459

Purpose

An AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancer

Depositor

Gordon Fishell

Article

Wu et al bioRxiv 2024.07.20.604399

Insert

ChR2-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1290-AAV-dSaCas9

Plasmid

#223139

Purpose

AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RB1

Plasmid

#222550

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RB1 (RB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KDM5B

Plasmid

#222555

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of KDM5B

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

KDM5B (KDM5B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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LAMTOR1-APEX2

Plasmid

#215556

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMTOR1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-human p53 1-50 (HK180)

Plasmid

#206458

Purpose

p53 1-50 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

p53 1-50 (TP53 Human)

Use

Tags

PH mcherry

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC8-pA

Plasmid

#220760

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC8

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC6-pA

Plasmid

#220770

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

oScarlert-WPRE-BC6

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC6-pA

Plasmid

#220758

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC6

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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