We narrowed to 8,518 results for: sgRNA

1. p8270 LentiCRISPR v2 hygro sgSIGIRR-3

   Plasmid

   #193979

   Purpose

   Expression of spCas9 and sgRNA targeting SIGIRR

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDIV272

   Plasmid

   #226183

   Purpose

   Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1p (Komagataella phaffii)

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMP8835

   Plasmid

   #220902

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP8921

   Plasmid

   #220903

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJMP8923

   Plasmid

   #220904

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJMP8932

   Plasmid

   #220906

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hLig4 guide2 in pX458

   Plasmid

   #211536

   Purpose

   sgRNA-2 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. hLig4 guide1 in pX458

   Plasmid

   #211535

   Purpose

   sgRNA-1 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR-TC9

   Plasmid

   #202088

   Purpose

   entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

   Depositor

   David Edgell

   Article

   Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

   Insert

   TevCas9 + sgRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

    Plasmid

    #193980

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT-GFP-rG1

    Plasmid

    #188967

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtggaaaacaatgcgaccgactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT-GFP-rG2

    Plasmid

    #188968

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtccatgtaatcagcgtctactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PHY55-mouse U6-sgLMNA

    Plasmid

    #164045

    Purpose

    U6-driven sgRNA targeting LMNA

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    LMNA targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJJF496_dpy-10_CDS_sg6

    Plasmid

    #164267

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJF495_dpy-10_CDS_sg6

    Plasmid

    #164268

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJF449_dpy-10_CDS_sg1

    Plasmid

    #163866

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJJF328_sqt-3_3′UTR_sg2

    Plasmid

    #163867

    Purpose

    sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the 3'UTR of sqt-3
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGuide-P1-O1a (EL702)

    Plasmid

    #140040

    Purpose

    CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

    Depositor

    Johan Elf

    Article

    Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

    Insert

    barcode P1 and sgRNA lacO1 array
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgPYM1

    Plasmid

    #105249

    Purpose

    human PYM1 sgRNA

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    sgRNA for PYM1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only