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Plasmid#191744PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mRuby3ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mNeptune2.5
Plasmid#191748PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mNeptune2.5ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Cas9
Plasmid#186723PurposeTransient expression of Cas9 in plant cells.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTurbo-mito
Plasmid#187177PurposeExpress mitochondira-localized miniTurbo under the control of CAG promoterDepositorInsertmtActA fused with miniTurbo (BirA mutant)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mTurbo
Plasmid#187178PurposeExpress histone H2B tagged with miniTurbo under the control of CAG promoterDepositorInserthistone H2B fused with miniTurbo (BirA mutant)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-10xSunTag (SRv1.1)
Plasmid#185798Purposepcp-10xSunTag component for SunRISER SRv1.1DepositorInsertpcp-10xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ubc-nls-pcp-12xSunTag (SRv1.2)
Plasmid#185799Purposepcp-12xSunTag component for SunRISER SRv1.2DepositorInsertpcp-12xSunTag
UseTagsFactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-iRFP670-tDeg
Plasmid#185403PurposeiRFP670-tDeg fluorogenic proteinDepositorInsertiRFP670-tDeg
UseTagsExpressionMammalianMutationWTPromoterminiCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-mCerulean-tDeg
Plasmid#185401PurposemCerulean-tDeg fluorogenic proteinDepositorInsertmCerulean-tDeg
UseTagsExpressionMammalianMutationWTPromoterminiCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-MiniE-mEm
Plasmid#181991PurposeMiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporterDepositorInsertH2B-MiniE-mEm
UseTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-QF2-MCS-polyA
Plasmid#174026PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-QF2
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterEc-TTL-P112 and Ec-TTL-P104Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
UseTagsExpressionBacterialMutationPromoterEc-TTL-P112 and BBa_J23116Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD
Plasmid#162814PurposeSTELLA plasmid for amber suppression of C-terminally labelled SMIM38DepositorInsertSMIM38(C-TAG)-Intein-DD
UsePiggybacTagsExpressionMammalianMutationSMIM38(Ochre52,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI
Plasmid#162803PurposeControl STELLA destination plasmid for N-terminally labelled proteinsDepositorInsertUb(G75,76A)-BsmBI
UsePiggybacTagsExpressionMammalianMutationUbiquitin (G75,76A)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*SMIM38
Plasmid#162808PurposeSTELLA plasmid for amber suppression of N-terminally labelled SMIM38DepositorInsertUb-(N-TAG)SMIM38
UsePiggybacTagsExpressionMammalianMutationSMIM38 (Met1, Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4x7SKPylT_EF1_Ub-*PIGBOS
Plasmid#162809PurposeSTELLA plasmid for amber suppression of N-terminally labelled PIGBOSDepositorInsertUb-(N-TAG)PIGBOS
UsePiggybacTagsExpressionMammalianMutationPIGBOS (Met1, Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG369-lyn11-redFAST
Plasmid#160356PurposeExpresses Lyn11-redFAST in mammalian cellsDepositorInsertlyn11-redFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG361-lyn11-greenFAST
Plasmid#160352PurposeExpresses Lyn11-greenFAST in mammalian cellsDepositorInsertlyn11-greenFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW294
Plasmid#139491PurposeInsertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGRE2 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGRE2Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only