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Plasmid#154327PurposesgRNA Target Vector for CRISPRDepositorInsertttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1851
Plasmid#154329PurposesgRNA Target Vector for CRISPRDepositorInsertcxTi10882 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1884
Plasmid#154332PurposesgRNA Target Vector for CRISPRDepositorInsertcxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)PromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK1520_puroR
Plasmid#173901PurposeSpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMV/U6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ210
Plasmid#109329PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selectionDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds
Plasmid#119059PurposeFluorescent vector with zebrafish pax3a enhancer 5 (strong activity)DepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1pr_v2-RT
Plasmid#97088PurposeEncodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.DepositorInsertNEAT1pr_v2-RT (NEAT1 Human)
UseCRISPRTagsEYFPExpressionMutationPromoterCMVAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1m_v1-RT
Plasmid#97089PurposeEncodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.DepositorInsertNEAT1m_v1-RT (NEAT1 Human)
UseCRISPRTagsEYFPExpressionMutationPromoterCMVAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-HK2-3xFLAG
Plasmid#163451Purposelentiviral overexpression vector for human HK2DepositorInsertHK2 (HK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GPT1-3xFLAG
Plasmid#163448Purposelentiviral overexpression vector for human GPT1DepositorInsertGPT1 (GPT Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-gRNA
Plasmid#65626PurposeEmpty vector for the expression U6 driven gRNADepositorInsertU6-gRNA
UseTagsExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBA1018
Plasmid#189587PurposeT4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)DepositorInsertT4wtGT7 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of T4 from 52.1 (part…PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-2x
Plasmid#164414PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgGFP
Plasmid#190899PurposeAAV vector expressing sgGFPDepositorInsertsgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-GPT1-3xFLAG
Plasmid#163446Purposelentiviral overexpression vector for human GPT1DepositorInsertGPT1 (GPT Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only