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Plasmid#228721PurposeExpresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2 nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R
Plasmid#228727PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2703-CMV-iNme2Smu-ABE8e-nt
Plasmid#228724PurposeExpresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2Smu nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R
Plasmid#228728PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R
Plasmid#228732PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2712-CMV-eNme2-C-ABE8e-i1-L10
Plasmid#228733PurposeExpresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid eNme-C nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R
Plasmid#228731PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-MYO1C
Plasmid#227305PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of MYO1C (MYO1C Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-PXN
Plasmid#227316PurposeDonor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorArticleInsertPXN Homology Arms flanking a mStayGold Tag (PXN Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-moxGFP
Plasmid#227273PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorArticleInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-mScarlet
Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorArticleInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-CETN2
Plasmid#227291PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of CETN2 (CETN2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-CENPA
Plasmid#227282PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of CENPA (CENPA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3428 MTK234-LacO-BtoApegRNA
Plasmid#226669PurposeMTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNADepositorInserthU6-LacOS-BtoApegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-LacOSAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3331 MTK234-TetO-AtoBpegRNA
Plasmid#226668PurposeMTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNADepositorInserthU6-TetO-AtoBpegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-TetOAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3478 PB-PEmax-mTagBFP2
Plasmid#226664PurposePiggyBac cargo vector encoding PEmax and mTagBFP2DepositorInsertsPEmax
mTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and UBCAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3241 PB-AtoBpegRNA-mCherry
Plasmid#226666PurposePiggyBac cargo vector encoding A to B pegRNA marked with mCherryDepositorInsertAtoB pegRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3401 BtoApegRNA-sfGFP
Plasmid#226667PurposePiggyBac cargo vector encoding B to A pegRNA marked with sfGFPDepositorInsertBtoA pegRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only