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Plasmid#195988PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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pDrVSP-IRES2-EGFP
Plasmid#80333PurposeExpresses Danio rerio VSP with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST exorh:mCherry
Plasmid#195989PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ING2
Plasmid#109502PurposeMuscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsertactc1b-mKate2-ING2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD
Plasmid#37275PurposeTALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)DepositorTypeEmpty backboneUseTagsFlag, Fok I DD, Tal-C, and Tal-NExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway
Plasmid#37846DepositorTypeEmpty backboneUseZebrafish enhancer assay vectorTagsExpressionMutationPromoterE1bAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTransgenesis p3 sv40 minimal promoter:: Katushka RFP
Plasmid#131731PurposeTransposon based transgenesisDepositorInsertSV40MinPromoter
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_PP1-mCitrine
Plasmid#172472PurposeMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.DepositorInsertprolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
UseLentiviralTagsmCitrine and prolactin signal sequence (cleaved p…ExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECEbasp
Plasmid#132968Purposegata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6) with a b-actin basal promoter
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-ctrl-guides
Plasmid#168240Purpose"neutrophil specific GFP with ubiquitous ctrl sgRNAs"DepositorInsertcontrol sgRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag-P2A-QFGal4-SV40pA
Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_Z3_Gal4VP64
Plasmid#169916PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4P1Egfp
Plasmid#90160Purposecontains dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PUC57_BAG3P209L-eGFP
Plasmid#98183PurposeCodes for full length, GFP tagged mutant Bag3DepositorInsertBAG3P209L-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationP209LPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2P1Egfp
Plasmid#90162Purposecontains lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE1-P1Egfp
Plasmid#90146Purposecontains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E1P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2CE1-P1Egfp
Plasmid#90153Purposecontains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2E1P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1
Plasmid#109529PurposeMuscle specific zebrafish expression of mKate2 fused to Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only