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Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4P1Egfp
Plasmid#90160Purposecontains dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_Z3_Gal4VP64
Plasmid#169916PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2P1Egfp
Plasmid#90162Purposecontains lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ING2
Plasmid#109502PurposeMuscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsertactc1b-mKate2-ING2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-2PH-PLCd-mKate2
Plasmid#109500PurposeMuscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.DepositorInsertactc1b-2PH-PLCd-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PUC57_BAG3P209L-eGFP
Plasmid#98183PurposeCodes for full length, GFP tagged mutant Bag3DepositorInsertBAG3P209L-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationP209LPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE1-P1Egfp
Plasmid#90146Purposecontains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E1P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2CE1-P1Egfp
Plasmid#90153Purposecontains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2E1P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1
Plasmid#109529PurposeMuscle specific zebrafish expression of mKate2 fused to Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
782 pMEActMEK
Plasmid#31166DepositorInsertMEK (MAP2K1 Human)
UseEntry vectorTagsExpressionMutationSer218Glu Ser222Asp; activated MEKKPromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F2bbs kan GCANNNAGGk
Plasmid#18752DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pME-foxc1a
Plasmid#132971Purposefull-length of foxc1a without stop codon in pmE Gateway vectorDepositorInsertfoxc1a (foxc1a Zebrafish)
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Dusp6_2KBpromoter
Plasmid#32666DepositorInsertDusp6 (dusp6 Zebrafish)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3E-rev_U6_MCS
Plasmid#109769PurposeMultisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequenceDepositorInsertU6-gRNA cassette
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1-DN
Plasmid#109530PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
ath5:H2B-RFP
Plasmid#105960Purposetransgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 constructDepositorInsertH2B (H2BC21 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-LactC2
Plasmid#109499PurposeMuscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertactc1b-mKate2-LactC2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E2P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only