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Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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pMIY-Arg1-Flag
Plasmid#34581DepositorInsertArg1 (Arg1 Mouse)
UseRetroviralTagsFLAG and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-GFP
Plasmid#66080PurposeThis G protein alpha-q construct contains internal insertions of GFP and the EE epitopeDepositorInsertG protein alpha-q-EE-YFP (Gnaq Mouse)
UseTagsGFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP FAK Y397F
Plasmid#50516PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase, mutated
UseTagsGFPExpressionMammalianMutationY397FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK GFP Paxillin
Plasmid#50529PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertPaxillin (PXN Human)
UseTagsGFP21ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS2-123
Plasmid#26598DepositorInsertSynthetic
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGFP Cortactin
Plasmid#50728Purposemammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCortactin (CTTN Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
cdk1 FRET sensor (2327)
Plasmid#26064DepositorInsertCdk1 FRET Sensor
UseTagsCFP variant, FLAG, and YFP variantExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGFP CrkII
Plasmid#50730Purposemammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCrkII (CRK Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNC891
Plasmid#20397DepositorInsertYFP-reporter
UseTagsExpressionYeastMutationX=M (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHZ054
Plasmid#27505DepositorInsertpmyo-3::yfp::lin-14_3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNS2-sigmaVL
Plasmid#26756DepositorInsertCFP, mCherry, and cI-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)
Plasmid#40999DepositorTypeEmpty backboneUseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBNJ28.2
Plasmid#66874PurposeFor transposon tagging with YFPDepositorInserteYFP in a transposon
UseSource for a tn7 transposonTagseYFPExpressionMutationPromoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNNS2-ySEc
Plasmid#26757DepositorInsertYFP, CFP and gal promoters
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-nLuc
Plasmid#199755PurposeControl construct of elongation reporter to quantify elongation of YFPDepositorInsertNanoluc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAX-SCLM
Plasmid#216757PurposeFor strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoterDepositorInsertSuperClomeleon
UseTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCAG (cytomegalovirus-chicken beta-actin-rabbit be…Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K27 histone methylation reporter
Plasmid#22865DepositorInsertK27 HMR
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K9 histone methylation reporter
Plasmid#22866DepositorInsertK9 HMR
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only