Purpose
CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
Inserts
Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
Use
Toxoplasma gondii expression
Tags
Expression
Mutation
Note: A S245F mutation is present but did not app…
Promoter
DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
Available since
Oct. 4, 2021
Availability
Academic Institutions and Nonprofits only