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Plasmid#174423PurposeExpresses human Drp1 Isoform 3 with K38A mutation in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK38APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
UseTags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEn-C1.1
Plasmid#61479PurposeEncodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPCDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-dDrp1-StrepII
Plasmid#174421PurposeExpresses D. melanogaster Drp1 in bacteriaDepositorInsertDrp1 (Drp1 Fly)
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK317
Plasmid#72472Purposepuc118 modified to contain ribosome binding siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterlacAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUNos_C1
Plasmid#33297DepositorTypeEmpty backboneUsePlant expressionTagsExpressionMutationPromotermaize ubiquitin (Ubi-1)Available sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFloxed DHFR-TS*
Plasmid#100605PurposepUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTRDepositorInsertFloxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rich-pT7
Plasmid#108445PurposeThis plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterpT7Available sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLO5.01
Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#113252PurposepUC19 backbone with E. coli CRISPR arrayDepositorInsertE. coli CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW091
Plasmid#113253PurposepUC19 backbone with S. pyogenes CRISPR arrayDepositorInsertS. pyogenes CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK601
Plasmid#109070PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_03784
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK586
Plasmid#109069PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_05705
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK583
Plasmid#109072PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_10057
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK600
Plasmid#109068PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_02256
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCVD432
Plasmid#63755PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.DepositorInsertEAggEC
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rp51a Hyperstabilized 5'SS
Plasmid#100988PurposepUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGTDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut ATG/GTATGT -> AAG/GTAAGTPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only