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Plasmid#107470PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS477FPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(150-261)-RTA(125-190)
Plasmid#99674PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-P65(151-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI425V-myc
Plasmid#107464PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationI425VPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-1X snRP1
Plasmid#99687PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR-mOrange2
Plasmid#228608PurposeMammalian expression of mOrange2, for MuSIC barcode constructionDepositorInsertmOrange2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mBeRFP
Plasmid#228601PurposeMammalian expression of mBeRFP, for MuSIC barcode constructionDepositorInsertmBeRFP
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTagBFP2
Plasmid#228596PurposeMammalian expression of mTagBFP2, for MuSIC barcode constructionDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TJP1
Plasmid#227300PurposeDonor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)DepositorArticleInsertTJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-LSSmOrange
Plasmid#228600PurposeMammalian expression of LSSmOrange, for MuSIC barcode constructionDepositorInsertLSSmOrange
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTFP1
Plasmid#228602PurposeMammalian expression of mTFP1, for MuSIC barcode constructionDepositorInsertmTFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-CyOFP1
Plasmid#228604PurposeMammalian expression of CyOFP1, for MuSIC barcode constructionDepositorInsertCyOFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mRuby3
Plasmid#228609PurposeMammalian expression of mRuby3, for MuSIC barcode constructionDepositorInsertmRuby3
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mKate2
Plasmid#228610PurposeMammalian expression of mKate2, for MuSIC barcode constructionDepositorInsertmKate2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mBeRFP
Plasmid#228620PurposeMammalian expression of mBeRFP and extra insert, for MuSIC barcode constructionDepositorInsertmBeRFP
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mTFP1
Plasmid#228621PurposeMammalian expression of mTFP1 and extra insert, for MuSIC barcode constructionDepositorInsertmTFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-CyOFP1
Plasmid#228623PurposeMammalian expression of CyOFP1 and extra insert, for MuSIC barcode constructionDepositorInsertCyOFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mOrange2
Plasmid#228627PurposeMammalian expression of mOrange2 and extra insert, for MuSIC barcode constructionDepositorInsertmOrange2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby3
Plasmid#228628PurposeMammalian expression of mRuby3 and extra insert, for MuSIC barcode constructionDepositorInsertmRuby3
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R
Plasmid#228722PurposeExpresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2702-CMV-iNme2-ABE8e-nt
Plasmid#228723PurposeExpresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2 nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only