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Addgene
Showing: 201 - 220 of 935 results

  1. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  2. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

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  3. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21916-plasmid-map-sequence-id-358974

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  4. pCDH-CB-copGFP-T2A-iCre

    Plasmid
    #72256
    Purpose
    Express copGFP and iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-iCre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72256-plasmid-map-sequence-id-134470

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  5. pTJK477

    Plasmid
    #138005
    Purpose
    3xMYC-SIX2 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    3xMYC tagged SIX2 (SIX2 Human)
    Use
    Lentiviral
    Tags
    3xMYC
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138005-plasmid-map-sequence-id-291774

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  6. pTJK421

    Plasmid
    #138007
    Purpose
    BirA*-SIX2 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    BirA* tagged SIX2 (SIX2 Human)
    Use
    Lentiviral
    Tags
    BirA*
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138007-plasmid-map-sequence-id-291775

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  7. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

    Plasmid
    #154987
    Purpose
    Lentiviral vector expressing Human ACE2 and TMPRSS2
    Depositor
    Sonja Best
    Insert
    ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154987-plasmid-map-sequence-id-310100

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  8. pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

    Plasmid
    #154983
    Purpose
    Lentiviral vector expressing Human ACE2 and TMPRSS2
    Depositor
    Sonja Best
    Insert
    ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154983-plasmid-map-sequence-id-307933

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  9. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid
    #214468
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214468-plasmid-map-sequence-id-424065

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  10. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid
    #214471
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214471-plasmid-map-sequence-id-425103

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  11. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177435
    Purpose
    To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.
    Depositor
    David Andrews
    Insert
    Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177435-plasmid-map-sequence-id-348466

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  12. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Eleanor Chen
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83966-plasmid-map-sequence-id-160487

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  13. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220363-plasmid-map-sequence-id-436888

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  14. pLgw ELK1Y158A-V5-EcoDam

    Plasmid
    #98609
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98609-plasmid-map-sequence-id-189409

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  15. pLgw EcoDam-V5-ELK1Y158A

    Plasmid
    #98608
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98608-plasmid-map-sequence-id-189405

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  18. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196715-plasmid-map-sequence-id-389692

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  19. pTJK438

    Plasmid
    #138004
    Purpose
    3xMYC-SIX1 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    3xMYC tagged SIX1 (SIX1 Human)
    Use
    Lentiviral
    Tags
    3xMYC
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138004-plasmid-map-sequence-id-274998

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Showing: 201 - 220 of 935 results