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Plasmid#173004Purposefirefly luciferase reporter vector with 12x clustered TEAD binding sitesDepositorInsert12xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Ef1a_DIO_hTyrHA_minBack
Plasmid#205286PurposeInduces NM-like pigment accumulation in catecholaminergic neuronsDepositorInserthTyrosinase
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Flex_syn_SpikeyGi2_kv2.1
Plasmid#195314PurposeGenetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSynDepositorInsertSpikeyGi2
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin1_NOPLight1
Plasmid#195580PurposeExpresses NOPLight1 in neuronsDepositorInsertNOPLight1
UseAAVTagsExpressionMammalianMutationPromoterhuman Synapsin-1Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_VP1_RaPID_r1c3
Plasmid#65723PurposeProduce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 onlyDepositorInsertmCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_AAVS1
Plasmid#141212PurposepDECKO_mCherry_AAVS1_186. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GWRev_Dest
Plasmid#86954PurposeGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.ArcLightD.WPRE.SV40
Plasmid#100037PurposeAAV expression of ArcLightD driven by hSynap promoterDepositorInsertArcLightD
UseAAVTagsExpressionMammalianMutationPromoterhSynapAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2
Plasmid#79902PurposeGene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor LocusDepositorInsert3xFLAG_Twin_Strep_EZH2
UseCRISPR and TALENTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterPGK1Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_AAVS1
Plasmid#184487PurposeLentivirus all in one CRISPR vector targeting human AAVS1 geneDepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR.R205A
Plasmid#112162PurposeGABA SensorDepositorInsertiGABASnFR.R205A
UseAAVTagsExpressionMutationR205A (no GABA binding)PromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Cbh_DIO_hTyr
Plasmid#205287PurposeInduces NM-like pigment accumulation in catecholaminergic neuronsDepositorInserthTyrosinase
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.iGABASnFR.F102Y.Y137L
Plasmid#112174PurposeGABA SensorDepositorInsertiGABASnFR.F102Y.Y137L
UseAAVTagsExpressionMutationF102Y.Y137LPromoterGFAPAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR.F102Y.Y137L
Plasmid#112161PurposeGABA SensorDepositorInsertiGABASnFR.F102Y.Y137L
UseAAVTagsExpressionMutationF102Y.Y137LPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1
Plasmid#141211PurposepDECKO_GFP_AAVS1_1801. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
psAAVS1-T2
Plasmid#60543PurposeGFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activitiesDepositorInsertProtoSpacer AAVS1-T2 (AAVS1 Human)
UseCRISPR; KanTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1A
Plasmid#183337PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2
Plasmid#171938PurposeExpresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteinsDepositorInsertApex2, mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_AAVS1_1801
Plasmid#159090Purposetargeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only