Addgene

We narrowed to 241 results for: Weissman Jonathan

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mCRISPRa-v2, Stress and Proteostasis (m3), top 5 sgRNAs/gene

Pooled Library

#84000

Purpose

Mouse CRISPRi Pooled Library targeting stress and proteostasis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to U87

Pooled Library

#86542

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
mCRISPRa-v2, Unassigned (m7), top 5 sgRNAs/gene

Pooled Library

#84004

Purpose

Mouse CRISPRa Pooled Library targeting unassigned genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to MCF7

Pooled Library

#86546

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to HEK293T

Pooled Library

#86543

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - U87 & HEK293T

Pooled Library

#86547

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - K562 & HeLa

Pooled Library

#86549

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, Unassigned (h7), top 5 sgRNAs/gene

Pooled Library

#83977

Purpose

Human CRISPRi Pooled Library targeting unassigned genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - K562, HeLa, & MCF7

Pooled Library

#86548

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to HeLa

Pooled Library

#86545

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

Pooled Library

#136479

Purpose

Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

Depositor

Jonathan Weissman

Article

Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

Pooled Library

#136478

Purpose

Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

Depositor

Jonathan Weissman

Article

Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pPS

Plasmid

#85801

Purpose

Backbone for pooled cloning of Perturb-seq libraries

Depositor

Aviv Regev

Article

Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pUS

Plasmid

#153416

Purpose

Backbone for cloning downstream of UCOE-SFFV

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge