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Plasmid#57214PurposeLocalization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511DepositorInsertActin (ACTB Human)
UseTagsPS-CFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MET FLAG46QDProCFPp303
Plasmid#15589DepositorInserthtt 46Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mECFP
Plasmid#223954PurposeGateway cloning of genes of interest with an N-terminal V5-mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mECFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eCFP
Plasmid#202142PurposeLevel 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteCFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENeCFPCNAL2 pc922
Plasmid#167563PurposeFor construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Landing_Pad_8xCTS-ECFP-pA
Plasmid#200242PurposeBackbone for cloning 8xCTSs (8xCRISPR-target sites)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA5_8xCTS-ECFP-pA
Plasmid#200247PurposeMammalian transfections; 8xCTS-ECFP reporter construct 5DepositorInsertsgRNA5_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_8xCTS-ECFP-pA
Plasmid#200245PurposeMammalian transfections; 8xCTS-ECFP reporter construct 3DepositorInsertsgRNA3_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_8xCTS-ECFP-pA
Plasmid#200244PurposeMammalian transfections; 8xCTS-ECFP reporter construct 2DepositorInsertsgRNA2_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNC-CFP-N
Plasmid#193410PurposeNimble cloning-compatible vector for protein subcellular localization in plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNC-CFP-C
Plasmid#193411PurposeNimble cloning-compatible vector for protein subcellular localization in plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbm5En2E4PCh(#251)
Plasmid#184083Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbiG4FER4P2A(#249)
Plasmid#184070Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2-P2A
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nCFP
Plasmid#185360PurposeFor mammalian expression of N-term CFP for split-CFPDepositorInsertCFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cCFP
Plasmid#185361PurposeFor mammalian expression of C-term CFP for split-CFPDepositorInsertCFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/eCFP-C
Plasmid#183156PurposePlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFPDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP3
Plasmid#129506PurposeExpresses AvicFP3 constitutively in E. coli (most strains)DepositorInsertAvicFP3
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP-tTA-ER-C3
Plasmid#134569PurposeExpresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion proteinDepositorInserttTA-ER
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-ECFP
Plasmid#118854Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-ECFP
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ECFP-TRAF1
Plasmid#111198Purposefluorescent fusion proteinDepositorInsertTRAF1 (TRAF1 Human)
UseTagsECFP and FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only