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Addgene
Showing: 201 - 220 of 1036 results
  1. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  2. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. pSPYngK+-hp

    Plasmid
    #48121
    Purpose
    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Insert
    codon optimized signal peptide of YngK
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pBP-PliaG

    Plasmid
    #112777
    Purpose
    Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage
    Depositor
    Insert
    PliaG promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pBP-ORF-xylE

    Plasmid
    #112780
    Purpose
    Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.
    Depositor
    Insert
    xylE open reading frame
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pTU1-D-recJD

    Plasmid
    #112784
    Purpose
    Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance
    Depositor
    Insert
    recJD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pTU1-A-yckABU

    Plasmid
    #112789
    Purpose
    Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis
    Depositor
    Insert
    yckABU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pIA028

    Plasmid
    #200419
    Purpose
    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
    Type
    Empty backbone
    Use
    Tags
    PAmCherry1
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pIA033

    Plasmid
    #200420
    Purpose
    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
    Type
    Empty backbone
    Use
    Tags
    bsPAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Type
    Empty backbone
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pBP-P43

    Plasmid
    #112775
    Purpose
    Strong promoter activity during exponential and stationary phase
    Depositor
    Insert
    P43 promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pBP-Pveg

    Plasmid
    #112776
    Purpose
    Strong promoter active during sporulation and vegetative growth
    Depositor
    Insert
    Pveg promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pBP-Pspac

    Plasmid
    #112778
    Purpose
    Promoter repressed by LacI (not natively produced in B. subtilis 168)
    Depositor
    Insert
    Pspac promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  14. pBP-Pars

    Plasmid
    #112779
    Purpose
    Promoter of ars operon. Repressed by ArsR, induced by arsenate or arsenite
    Depositor
    Insert
    Pars promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  15. pTU1-A-recJU

    Plasmid
    #112783
    Purpose
    Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance
    Depositor
    Insert
    recJU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 220 of 1036 results