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Plasmid#152459PurposeMammalian expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;CBPExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046272
Plasmid#145421PurposeYeast Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
YCe2721 HC_Kan_conn_W-Z
Plasmid#100664PurposeEMMA toolkit Assembly connector W-ZDepositorInsertEMMA toolkit Assembly connector W-Z
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG272-eya
Plasmid#99085PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)DepositorInserteyes absent (eya)
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_puro-mKate-lox2272
Plasmid#162076Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox2272
Plasmid#162078Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_blast-tagBFP-lox2272
Plasmid#162071Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
3410 pBM272 Bcl-XL
Plasmid#8772DepositorInsertBcl-XL (Bcl2l1 Mouse)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhyB (GB2721)
Plasmid#160603Purposechimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.DepositorInsertPhyB
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272.Hygro
Plasmid#22731DepositorInsertLox71-FRT-PGK-hygro-FRT-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE. The vec…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
3396 pBM272 Bax
Plasmid#8770DepositorInsertBax (Bax Mouse)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
sv272-HisMBP-TZAPZNF9-11
Plasmid#87185Purposebacterial expression of HisMBP-TZAPZNF9-11DepositorInsertTZAP (ZBTB48 Human)
UseTagsHisMBPExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDK1 gRNA (BRDN0001149272)
Plasmid#77390Purpose3rd generation lentiviral gRNA plasmid targeting human CDK1DepositorInsertCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-1 Jip4 (1-272)
Plasmid#59002PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsGstExpressionBacterialMutationJip4 (aa1-272)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-272)
Plasmid#58928PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-272aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP9B(E272Q)-HA
Plasmid#209257PurposeMammalian expression of ATP9B mutant E272QDepositorInsertATP9B (ATP9B Human)
UseRetroviralTagsHAExpressionMutationE272QPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-K272A
Plasmid#222215PurposeExpress DDX53 mutant K272A protein in bacteriaDepositorInsertDDX53_K272A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-K272A
Plasmid#222242PurposeExpress FLAG tagged DDX53 mutant K272A in mammalian cellsDepositorInsertDDX53 (DDX53 Human)
UseTags3xFLAGExpressionMammalianMutationK272APromoterT7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_EXOSC6:1-272
Plasmid#211084PurposeMammalian expression of full-length human EXOSC6. C-terminal NanoLuc luciferase tag.DepositorInsertEXOSC6:M1-P272 (EXOSC6 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only