1. p426 25Q GPD

   Plasmid

   #1181

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pBiT3.1-C_NEDD1:1-660

   Plasmid

   #211181

   Purpose

   Mammalian expression of full-length human NEDD1. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   NEDD1:M1-F660 (NEDD1 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQLinkN

   Plasmid

   #13670

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQLinkH

   Plasmid

   #13667

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   His, TEV, and attB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQLinkG2

   Plasmid

   #13671

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   GST, TEV, and attB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQLinkHD

   Plasmid

   #13668

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   Gateway cassette
   

   Use

   Co-expression

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQLinkGD

   Plasmid

   #13673

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   Gateway cassette
   

   Use

   Co-expression

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HaloTag-G protein gamma-11

   Plasmid

   #67042

   Purpose

   G protein gamma-11 tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-G protein gamma-11 (GNG11 Bovine)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LRP4-Flag/pCDNA3.1

   Plasmid

   #197291

   Purpose

   Plasmid expressing an antigen targeting LRP4 (extracellular)

   Depositor

   Steve Burden

   Insert

   LRP4 (Lrp4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEQ111m

   Plasmid

   #40190

   Depositor

   Paul Modrich

   Article

   Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

   Insert

   EcoRI endonuclease & EcoRI methylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Glutamic Acid 111 to Glutamine

   Promoter

   Lambda PL

   Available since

   Nov. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

10. FUGW-APOE-E4-T2A-mCherry

    Plasmid

    #190809

    Purpose

    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E4

    Promoter

    Ubi C

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUGW-APOE-E3-T2A-mCherry

    Plasmid

    #190808

    Purpose

    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E3

    Promoter

    Ubi C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUGW-APOE-E2-T2A-mCherry

    Plasmid

    #190807

    Purpose

    Express APOE E2 allele from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E2

    Promoter

    Ubi C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUGW-APOE-E4-T2A-Puro

    Plasmid

    #190810

    Purpose

    Express APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E4

    Promoter

    Ubi C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTet-O-APOE-E4-T2A-Puro

    Plasmid

    #190813

    Purpose

    Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E4

    Promoter

    Tet-O

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTet-O-APOE-E3-T2A-Puro

    Plasmid

    #190812

    Purpose

    Express APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E3

    Promoter

    Tet-O

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTet-O-APOE-E2-T2A-Puro

    Plasmid

    #190811

    Purpose

    Express APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E2

    Promoter

    Tet-O

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPH6

    Plasmid

    #40315

    Depositor

    Shaun Lott

    Article

    Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

    Insert

    Peptide:N-glycosidase F
    

    Use

    Tags

    HexaHistidine and OmpA3 leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. APOE_Halo_C_allele

    Plasmid

    #178121

    Purpose

    Donor vector for endogenous tagging of human APOE at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (APOE Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEQ1007

    Plasmid

    #83938

    Purpose

    express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

    Insert

    IRS1
    

    Use

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    R833L in IRS1 (please see depositor comment)

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge