Showing: 201 - 220 of 309 results
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Plasmid#65559PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1d834-1363-Poly(A)
Plasmid#65560PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationdel(834-1363)PromoterAvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1d834-1053-Poly(A)
Plasmid#65561PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationdel(834-1053)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8A-no stop
Plasmid#67333PurposehArl8a without stop codon in Gateway Entry vectorDepositorInsertARL8A (ARL8A Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL8A-GFP
Plasmid#67403PurposeMammalian expression of hArl8 with C-term GFP tagDepositorInsertARL8A (ARL8A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL8A-V5-His6
Plasmid#67424PurposeExpression of hArl8 with C-term V5 and His6 tag in E. coliDepositorInsertARL8A (ARL8A Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL8A-V5-His6
Plasmid#67445PurposeMammalian expression of hArl8 with C-term V5 and His6 tagDepositorInsertARL8A (ARL8A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL8A
Plasmid#67466PurposeMammalian expression of hArl8 with stop codon (native expression)DepositorInsertARL8A (ARL8A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Unc5b.a-Fc-His
Plasmid#72178PurposeExpresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5b.a (Unc5b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5B-FLAG
Plasmid#72195PurposeExpresses full-length Unc5b with a C-terminal FLAG tagDepositorInsertUnc5b (Unc5b Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027
Plasmid#134611PurposeMammalian expression of mouse KIF5A 378-1027DepositorInsertKIF5A (Kif5a Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD∆cat
Plasmid#81054Purposebacterial expression of catalytic dead catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057 with histid…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s_K852R pc3914
Plasmid#201704PurposeExpresses of GFP-tagged mTet1s_K852R in mammalian cellsDepositorInsertTet1s-K852R (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationLysine 852 mutated to argininePromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s_K852E pc3915
Plasmid#201705PurposeExpresses of GFP-tagged mTet1s_K852E in mammalian cellsDepositorInsertTet1s-K852E (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationLysine 852 mutated to glutamatePromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pmch-mTet1-ZF-CD pc3956
Plasmid#201709PurposeExpresses of mcherry-tagged mTet1-ZF-CD in mammalian cellsDepositorInsertTet1-ZF_CD (Tet1 Mouse)
UseTagsmCherryExpressionMammalianMutationZinc finger domain of Tet1 fused to its catalytic…PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
UseTagsExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA
Plasmid#174895Purposeamber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAG 150TAA in sfGFPPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UUA)_EF1_MmaPylRS
Plasmid#174896PurposeMma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UCA)_EF1_MmaPylRS
Plasmid#174899PurposeMma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5A-Rigor
Plasmid#172202PurposeExpresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5A (Kif5a Mouse)
UseTagseGFPExpressionMammalianMutationChanged Glycine 235 to AlaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 201 - 220 of 309 results