Addgene

We narrowed to 309 results for: 10B

Showing: 201 - 220 of 309 results

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attB-GFP-Tet1-Poly(A)

Plasmid

#65559

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1d834-1363-Poly(A)

Plasmid

#65560

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1363)

Promoter

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1d834-1053-Poly(A)

Plasmid

#65561

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1053)

Promoter

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL8A-no stop

Plasmid

#67333

Purpose

hArl8a without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL8A-GFP

Plasmid

#67403

Purpose

Mammalian expression of hArl8 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL8A-V5-His6

Plasmid

#67445

Purpose

Mammalian expression of hArl8 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL8A

Plasmid

#67466

Purpose

Mammalian expression of hArl8 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Unc5b.a-Fc-His

Plasmid

#72178

Purpose

Expresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b.a (Unc5b Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5B-FLAG

Plasmid

#72195

Purpose

Expresses full-length Unc5b with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b (Unc5b Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD∆cat

Plasmid

#81054

Purpose

bacterial expression of catalytic dead catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Use

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057 with histid…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852R pc3914

Plasmid

#201704

Purpose

Expresses of GFP-tagged mTet1s_K852R in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852R (Tet1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to arginine

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852E pc3915

Plasmid

#201705

Purpose

Expresses of GFP-tagged mTet1s_K852E in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852E (Tet1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to glutamate

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pmch-mTet1-ZF-CD pc3956

Plasmid

#201709

Purpose

Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1-ZF_CD (Tet1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Zinc finger domain of Tet1 fused to its catalytic…

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Use

Tags

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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