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Plasmid#200497PurposeLentiviral vector expressing gRNA targeting human MRPL21DepositorInsertMRPL21(16) (MRPL21 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPS5A-PTP-3Xflag-16S rRNA Left_G1397N
Plasmid#189641Purpose16S rRNA Left_G1397NDepositorInsertTALE and DddAtox
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Kv1.2 K+ channel scFv [K14/16]
Plasmid#182064PurposeMammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.DepositorInsertrecombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-6-C,16-C
Plasmid#124694PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-16-1-3p
Plasmid#103270PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-16-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-16-1-3p target (MIR16-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-16-2-3p
Plasmid#103271PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-16-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-16-2-3p target (MIR16-2 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-16 3x FLAG
Plasmid#111966PurposeHuman HEATs 1-16 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-16 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-16 insertedPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
F. prausnitzii 16S toehold switch sensor
Plasmid#110705PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertF. prausnitzii 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
R. hominis 16S toehold switch sensor
Plasmid#110703PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertR. hominis 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. rectale 16S toehold switch sensor
Plasmid#110702PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. rectale 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. breve 16S toehold switch sensor
Plasmid#110701PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. breve 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. adolescentis 16S toehold switch sensor
Plasmid#110700PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. adolescentis 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. longum 16S toehold switch sensor
Plasmid#110699PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. longum 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. thetaiotaomicron 16S toehold switch sensor
Plasmid#110697PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. thetaiotaomicron 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-4GS-LaG-2
Plasmid#172764PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)DepositorInsertLaG-16-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-6-C, 16-C
Plasmid#124681PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7274 MSCV-P C-FlagHA 16 ∆DLYC
Plasmid#163304PurposeExpresses HPV16 E7 ∆DLYCDepositorInsertHPV16 E7 ∆DLYC
UseRetroviralTagsFlagHAExpressionMutation∆DLYCPromoterMSCV LTRAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-6-C, 16-C
Plasmid#124689PurposeTuning expression of PD-1DepositorInsertPD-L1 (CD274 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1 Cyclin D2 long-mut-miR15/16
Plasmid#21647DepositorInsertCyclin D2 long-mut-miR15/16 (CCND2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant miR15/16 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only